Welcome Guest Search | Active Topics |

Tag as favorite
SDS Page Gel Electrophoresis
Options
Previous Topic Next Topic
Sarah_4925
#1 Posted : Saturday, July 18, 2020 8:40:11 PM
Rank: Newbie

Groups: Registered
Joined: 5/3/2020
Posts: 5

Thanks: 0 times
Was thanked: 0 time(s) in 0 post(s)
Hello,
In the AAMC Section Bank for Bio/Biochem,in passage 3 question 20 it asks about the conditions that the gel electrophoresis should be performed for a protein. The answer is C)native, and description says, "Use of a denaturing agent will disrupt the interactions between monomers. Use of a reducing agent only will disrupt any disulfide bonds." However in the CC for Biochemistry on page 121 in class we annotated that the SDS-page would require a denaturing deturgent of the protein prior to running it on the gel, which confuses me as it seems to contradict what the AAMC question explains. Would you be able to clarify this as I think I am misunderstanding something.
Thank you very much!
Back to top
Report?
INSTR_Katerina_102
#2 Posted : Saturday, July 18, 2020 8:55:45 PM
Rank: Advanced Member

Groups: Registered
Joined: 6/24/2019
Posts: 250

Thanks: 0 times
Was thanked: 0 time(s) in 0 post(s)
Hi Sarah,

Could you post the full question for context? This is pretty dependent on what you want to do.

It is true that conventional SDS-PAGE runs denatured proteins on a gel because molecular conformation can affect how something runs, but there are also variants of SDS-PAGE that will look at native variants of proteins - and when you use one or the other is dependant on the situation.

My guess is that maybe you're looking at monomer vs dimer formation of a pair of proteins, and in this case if you denatured and broke apart the dimer, you wouldn't be able to distinguish a sample containing only monomer from a sample containing two monomers in a dimer, but I'd have to look at the full question to be sure.

Hope this helps!

Katt
Back to top
Report?
Users browsing this topic
Guest
Tag as favorite
You cannot post new topics in this forum.
You cannot reply to topics in this forum.
You cannot delete your posts in this forum.
You cannot edit your posts in this forum.
You cannot create polls in this forum.
You cannot vote in polls in this forum.

Clean Slate theme by Jaben Cargman (Tiny Gecko)
Powered by YAF | YAF © 2003-2009, Yet Another Forum.NET
This page was generated in 0.074 seconds.