Welcome Guest Search | Active Topics |

Tag as favorite
EK ICE 5
Divya_4978
#1 Posted : Tuesday, June 30, 2020 11:14:29 PM
Rank: Member

Groups: Registered
Joined: 6/3/2020
Posts: 14

Thanks: 0 times
Was thanked: 0 time(s) in 0 post(s)
Hello,

I was wondering if you could explain question 72 of passage 1


Thank you in advance.
INSTR_Katerina_102
#2 Posted : Thursday, July 02, 2020 4:38:43 PM
Rank: Advanced Member

Groups: Registered
Joined: 6/24/2019
Posts: 250

Thanks: 0 times
Was thanked: 0 time(s) in 0 post(s)
Hi,

Can you clarify, is this the question in EK ICE 4 regarding duplex PCR with the RNA from the TP53 gene?

Thanks!
Divya_4978
#3 Posted : Friday, July 03, 2020 12:46:50 AM
Rank: Member

Groups: Registered
Joined: 6/3/2020
Posts: 14

Thanks: 0 times
Was thanked: 0 time(s) in 0 post(s)
Yes sorry, question 72, ICE 4 passage 1

Thank you
INSTR_Katerina_102
#4 Posted : Saturday, July 04, 2020 3:03:11 AM
Rank: Advanced Member

Groups: Registered
Joined: 6/24/2019
Posts: 250

Thanks: 0 times
Was thanked: 0 time(s) in 0 post(s)
Hi,

This is a bit of a tough question.

You have been given only the RNA of the TP53 gene as stated in the question stem(here the implication is mRNA). This mRNA has been spliced at this point, removing introns and promotor sequences starting transciption.

To do this Duplex PCR sequencing technique , you need to have double stranded DNA, so you would have to get your DNA from the reverse transcription of the mRNA provided.

Because exons 3 and 5 are expressed in the mRNA, it is likely that mutations in both can be easily detected in Duplex PCR, eliminating B and C. Similarly, the DNA you get from reverse transcription also has an untranslated region, meaning D is unlikely as well.

However, the AT regions of DNA upstream of the promoter usually refer to a TATA box, which is part of the promoter region that is spliced out of mRNA - because our source of information is mRNA, we would not be able to see this region and therefore cannot comment on its variation, suggesting that A is the correct answer.

Please let me know if this is unclear, I hope this helps.
Users browsing this topic
Guest
Tag as favorite
You cannot post new topics in this forum.
You cannot reply to topics in this forum.
You cannot delete your posts in this forum.
You cannot edit your posts in this forum.
You cannot create polls in this forum.
You cannot vote in polls in this forum.

Clean Slate theme by Jaben Cargman (Tiny Gecko)
Powered by YAF | YAF © 2003-2009, Yet Another Forum.NET
This page was generated in 0.094 seconds.