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Conditioned media
Natasha_4902
#1 Posted : Tuesday, August 11, 2020 2:56:07 AM
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Hi,

In this paragraph:

Researchers inoculated cell media with each of three virulent S. pneumoniae strains (strains I, II, and III) to a final concentration of 1.25 × 109 CFU/ml, where CFU is colony-forming-units. After 7 h (during log-phase growth), the media was filtered to remove the bacterial cells. Then alveolar epithelial cells (AECs) were incubated in this “conditioned” media for 7 h. An assay was used to show localized expression of 53BP1 and/or γH2AX in the AECs using fluorescently tagged antibodies (Figure 1).

What does the conditioned media refer to? Does conditioned media mean with bacteria?

Thank you
INSTR_Katerina_102
#2 Posted : Wednesday, August 12, 2020 9:11:39 PM
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Hi Natasha,

Yes, I believe they are referring to the media in which the virulent S. pneumoniae was grown in.

Hope this helps,

Katt
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