Welcome Guest Search | Active Topics |

Tag as favorite
Laboratory Techniques and Question about ETC
Shahd_6465
#1 Posted : Monday, June 14, 2021 8:29:55 PM
Rank: Member

Groups: Registered
Joined: 5/3/2021
Posts: 20

Thanks: 0 times
Was thanked: 0 time(s) in 0 post(s)
Hello,

1. In lecture 4 Study Guide 1, question 94 asks about the colour if samples from both (the fasting and the fed mice) were run on the same microarray, wouldn’t that create a yellow colour since there will be both green (up-regulated genes) from the fed mice and red (down-regulated genes) from the fasting mice?

2. Why is oxygen consumption increased when the proton gradient in mitochondria is dissipated?

Thank you
INSTR_Molly_129
#2 Posted : Sunday, July 11, 2021 8:21:06 PM
Rank: Advanced Member

Groups: Registered
Joined: 5/23/2021
Posts: 64

Thanks: 0 times
Was thanked: 0 time(s) in 0 post(s)
Hi Shahd,

1. This was a tricky one. I looked into this a bit because I also originally thought the same as you did, and from what I found, insulin is made quite constantly even in fasting states, but its actual release from beta cells is controlled by sensing fed states. It makes sense since it would be more effective this way since insulin is actually made in many steps (pre-proinsulin, then proinsulin, then insulin, followed by secretion). Therefore I believe this was the reason that in fasting mice, it would still show up green since you are still synthesizing the protein.

2. This is a response of your body. When the proton gradient is dissipated, there is now not enough ATP being formed, and since your body really needs ATP and FAST, its going to want to undergo even more aerobic respiration, so it makes sense for oxygen consumption to increase.

Hope that helps!

Molly

Users browsing this topic
Guest
Tag as favorite
You cannot post new topics in this forum.
You cannot reply to topics in this forum.
You cannot delete your posts in this forum.
You cannot edit your posts in this forum.
You cannot create polls in this forum.
You cannot vote in polls in this forum.

Clean Slate theme by Jaben Cargman (Tiny Gecko)
Powered by YAF | YAF © 2003-2009, Yet Another Forum.NET
This page was generated in 0.044 seconds.