Welcome Guest Search | Active Topics |

Tag as favorite
Reasoning Lecture ii ICE
Options
Previous Topic Next Topic
Pathik_6407
#1 Posted : Tuesday, June 01, 2021 10:07:43 PM
Rank: Newbie

Groups: Registered
Joined: 4/14/2021
Posts: 1

Thanks: 0 times
Was thanked: 0 time(s) in 0 post(s)
I'm having some difficulty understanding why 18 is C in the Examkrackers Reasoning Lecture ii ICE. The western blot seems like the WT group has the 29 kDa and 35 kDa bands but the answers say it only has the 35 kDa. Could someone please explain how you are supposed to read the western blot diagrams?
Back to top
Report?
INSTR_Taylor_140
#2 Posted : Monday, June 14, 2021 4:51:47 AM
Rank: Member

Groups: Registered
Joined: 6/11/2021
Posts: 27

Thanks: 0 times
Was thanked: 0 time(s) in 0 post(s)
Hi Pathik!

Remember that the darker the smear, the more protein (in this case Aqp2) is present. You can see that there is a prominently dark streak at the 31 kDA form on the 3rd panel, and not particularly prevalent in the other two. This allows us to rule out A and B as an answer. Choice C tells us that 31 kDA form is present in the third panel (which we know to be true) and that 35-45kDA is expressed in the wild type, which you can see as the darkest smear on the first panel. While it is true that 29 may be present in the WT group, that is not one of the answers, and everything in C is correct so that is the best choice.

Hope that helps!
Taylor
Back to top
Report?
Users browsing this topic
Guest
Tag as favorite
You cannot post new topics in this forum.
You cannot reply to topics in this forum.
You cannot delete your posts in this forum.
You cannot edit your posts in this forum.
You cannot create polls in this forum.
You cannot vote in polls in this forum.

Clean Slate theme by Jaben Cargman (Tiny Gecko)
Powered by YAF | YAF © 2003-2009, Yet Another Forum.NET
This page was generated in 0.084 seconds.